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msample preparation system dna kit promega maxwell viral total nucleic acid extraction kit  (Promega)
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bamhi/ecori-digested total cellular dna  (METTLER TOLEDO)
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total human chromosome dna probes  (Oncor Inc)
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Spatial distribution of human <t>chromosome</t> territories in normal senescent fibroblast nuclei: Digital images (>50 nuclei) for each chromosome were analysed by a simple erosion analysis script ( ; ). The script divides the cell nuclei into five shells of equal area and measures the % of signal intensity from both chromosome signal and the <t>DNA</t> (DAPI). The % of chromosome signal is normalised by division of the % of DAPI in each of the eroded shells ( y -axis); and the shell numbers of 1–5 are on the x -axis. The error bars represent the standard error of mean (SEM). Shells 1 and 2 denote the nuclear periphery and shells 4 and 5 the nuclear interior. chromosomes as indicated above each graph in senescent nuclei as visualised by FISH and specific probes and erosion analysis. (A) Chromosome 1, (B) Chromosome 2, (C) Chromosome 3, (D) Chromosome 4, (E) Chromosome 5, (F) Chromosome 6, (G) Chromosome 7, (H) Chromosome 8, (I) Chromosome 9, (J) Chromosome 10, (K) Chromosome 11, (L) Chromosome 12, (M) Chromosome 14, (N) Chromosome 15, (O) Chromosome 16, (P) Chromosome 17, (Q) Chromosome 20, (R) Chromosome 21, (S) Chromosome 22, (T) Chromosome Y.
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total deoxyribonucleic acid (dna) extraction brazol  (LGC Biotecnologia)
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Spatial distribution of human <t>chromosome</t> territories in normal senescent fibroblast nuclei: Digital images (>50 nuclei) for each chromosome were analysed by a simple erosion analysis script ( ; ). The script divides the cell nuclei into five shells of equal area and measures the % of signal intensity from both chromosome signal and the <t>DNA</t> (DAPI). The % of chromosome signal is normalised by division of the % of DAPI in each of the eroded shells ( y -axis); and the shell numbers of 1–5 are on the x -axis. The error bars represent the standard error of mean (SEM). Shells 1 and 2 denote the nuclear periphery and shells 4 and 5 the nuclear interior. chromosomes as indicated above each graph in senescent nuclei as visualised by FISH and specific probes and erosion analysis. (A) Chromosome 1, (B) Chromosome 2, (C) Chromosome 3, (D) Chromosome 4, (E) Chromosome 5, (F) Chromosome 6, (G) Chromosome 7, (H) Chromosome 8, (I) Chromosome 9, (J) Chromosome 10, (K) Chromosome 11, (L) Chromosome 12, (M) Chromosome 14, (N) Chromosome 15, (O) Chromosome 16, (P) Chromosome 17, (Q) Chromosome 20, (R) Chromosome 21, (S) Chromosome 22, (T) Chromosome Y.
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Image Search Results


Spatial distribution of human chromosome territories in normal senescent fibroblast nuclei: Digital images (>50 nuclei) for each chromosome were analysed by a simple erosion analysis script ( ; ). The script divides the cell nuclei into five shells of equal area and measures the % of signal intensity from both chromosome signal and the DNA (DAPI). The % of chromosome signal is normalised by division of the % of DAPI in each of the eroded shells ( y -axis); and the shell numbers of 1–5 are on the x -axis. The error bars represent the standard error of mean (SEM). Shells 1 and 2 denote the nuclear periphery and shells 4 and 5 the nuclear interior. chromosomes as indicated above each graph in senescent nuclei as visualised by FISH and specific probes and erosion analysis. (A) Chromosome 1, (B) Chromosome 2, (C) Chromosome 3, (D) Chromosome 4, (E) Chromosome 5, (F) Chromosome 6, (G) Chromosome 7, (H) Chromosome 8, (I) Chromosome 9, (J) Chromosome 10, (K) Chromosome 11, (L) Chromosome 12, (M) Chromosome 14, (N) Chromosome 15, (O) Chromosome 16, (P) Chromosome 17, (Q) Chromosome 20, (R) Chromosome 21, (S) Chromosome 22, (T) Chromosome Y.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Interphase Chromosomes in Replicative Senescence: Chromosome Positioning as a Senescence Biomarker and the Lack of Nuclear Motor-Driven Chromosome Repositioning in Senescent Cells

doi: 10.3389/fcell.2021.640200

Figure Lengend Snippet: Spatial distribution of human chromosome territories in normal senescent fibroblast nuclei: Digital images (>50 nuclei) for each chromosome were analysed by a simple erosion analysis script ( ; ). The script divides the cell nuclei into five shells of equal area and measures the % of signal intensity from both chromosome signal and the DNA (DAPI). The % of chromosome signal is normalised by division of the % of DAPI in each of the eroded shells ( y -axis); and the shell numbers of 1–5 are on the x -axis. The error bars represent the standard error of mean (SEM). Shells 1 and 2 denote the nuclear periphery and shells 4 and 5 the nuclear interior. chromosomes as indicated above each graph in senescent nuclei as visualised by FISH and specific probes and erosion analysis. (A) Chromosome 1, (B) Chromosome 2, (C) Chromosome 3, (D) Chromosome 4, (E) Chromosome 5, (F) Chromosome 6, (G) Chromosome 7, (H) Chromosome 8, (I) Chromosome 9, (J) Chromosome 10, (K) Chromosome 11, (L) Chromosome 12, (M) Chromosome 14, (N) Chromosome 15, (O) Chromosome 16, (P) Chromosome 17, (Q) Chromosome 20, (R) Chromosome 21, (S) Chromosome 22, (T) Chromosome Y.

Article Snippet: Directly labelled total human chromosome DNA probes (Appligene Oncor) were denatured by incubating at 70°C for 10 min followed by 30 min reannealing at 37°C.

Techniques: